Journal: BMC Microbiology

Article Title: Reproductive tract microbiota dysbiosis in ovarian endometrioma and adenomyosis: multi-site 16S rRNA profiling and functional impact of key bacterial species on human endometrial stromal cells

doi: 10.1186/s12866-025-04412-7

Figure Lengend Snippet: Validation of key differentially expressed genes (DEGs) at mRNA and protein levels. A-B qRT-PCR validation of relative mRNA expression levels for selected genes (CEBPB, SERPINE1, S100A1, CKS1B, ADH1B, MYOM1, PTGFR, ACLY) in T-HESCs comparing AM vs. NC and CC vs. NC co-culture conditions. Expression normalized to GAPDH. C-D ELISA validation of protein concentrations for corresponding key molecules in cell culture supernatants, comparing AM vs. NC and CC vs. NC co-culture conditions. Data presented as mean ± SD or similar; significance indicated ( P < 0.05)

Article Snippet: Kits used included those for: ADH1B (MyBioSource, Cat. No. MBS4503934); MYOM1 (Thermo Fisher Scientific, Cat. No. PIPA5100626; or Proteintech, Cat. No. 22084-1-AP); PTGFR (ABclonal, Cat. No. RK07240; or MyBioSource, Cat. No. MBS9714736); ACLY (RayBiotech, Cat. No. ELH-ACLY; Biomatik, Cat. No. EKF60626 ; BPS Bioscience, Cat. No. 79904; or ABclonal, Cat. No. RK12483); CEBPB (Antibodies-online, Cat. No. ABIN989871); SERPINE1 (Proteintech, Cat. No. KE00109; MyBioSource, Cat. No. MBS175945; R&D Systems, Cat. No. DSE100; ABclonal, Cat. No. RK00133; or Sino Biological, Cat. No. KIT10296 ); S100A1 (Thermo Fisher Scientific, Cat. No. EH402RB; RayBiotech, Cat. No. ELH-S100A1; or MyBioSource, Cat. No. MBS4503934); and CKS1B (MyBioSource, Cat. No. MBS7207214; or Biomatik, Cat. No. EKA50789 ).

Techniques: Biomarker Discovery, Quantitative RT-PCR, Expressing, Co-Culture Assay, Enzyme-linked Immunosorbent Assay, Cell Culture